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I found this on YouTube and thought it would add to the theory discussions about temperament.
I can definitely hear the difference, the retuned guitar sounds 'calmer' to me, and regular tuning has a 'stress' to it that's hard to define... See what you think.

I think this was posted a few months ago Dean, we were all discussing it in detail.... if you look back in the threads you should find it...

Must admit it got a little abstruse for me and I tuned out (so to speak)!